keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
The cell-permeant pH indicator, carboxy SNARF-1, acetoxymethyl ester, acetate (CAS #126208-13-7) has a pKa of ~7.5 after de-esterification, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF-1 exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths – typically 580 nm and 640 nm – to be used for quantitative determinations of pH.
説明 | The cell-permeant pH indicator, carboxy SNARF-1, acetoxymethyl ester, acetate (CAS #126208-13-7) has a pKa of ~7.5 after de-esterification, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF-1 exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths – typically 580 nm and 640 nm – to be used for quantitative determinations of pH. |
In vitro | Incubating cells in 1~10 μM SNARF AM acetate for 30 minutes at the optimum temperature for the specific cell type of interest is recommend. Then, cells should be washed before pH measurements. |
分子量 | 567.55 |
分子式 | C32H25NO9 |
CAS No. | 126208-13-7 |
keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
You can also refer to dose conversion for different animals. 詳細
bottom
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
5-(and-6)-Carboxy SNARF-1, Acetoxymethyl Ester, Acetate 126208-13-7 5(and6)Carboxy SNARF1, Acetoxymethyl Ester, Acetate 5 (and 6) Carboxy SNARF 1, Acetoxymethyl Ester, Acetate Inhibitor inhibitor inhibit