Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Agerafenib (CEP32496) is a highly potent inhibitor of BRAF.
説明 | Agerafenib (CEP32496) is a highly potent inhibitor of BRAF. |
ターゲット&IC50 | c-Kit:2 nM(Kd), PDGFRβ:2 nM(Kd), Abl-1:3 nM(Kd), Lck:2 nM(Kd), RET:2 nM(Kd) |
キナーゼ試験 | Binding assay: Kinases are produced displayed on T7 phage or by expression in HEK-293 cells and tagged with DNA. Binding reactions are performed at room temperature for 1 hour, and the fraction of kinase not bound to test compound is determined by capture with an immobilized affinity ligand and quantitation by quantitative PCR. Each kinase is tested individually against CEP-32496. Kd values are determined using eleven serial 3-fold dilutions and presented as mean values from experiments performed in duplicate. Variability between individual values is less than 2-fold. |
細胞研究 | Cells are seeded at 104 cells per well in DMEM with 10% fetal calf serum and allowed to attach. The cells are washed with PBS and switched to DMEM with 0.5% of serum and incubated overnight. CEP-32496 is then added at various concentrations with a final DMSO concentration of 0.5% and incubated for 72 h. At the end of incubation, a Cell Titer Blue is added per instructions, and incubation is continued for 3 hours. Remaining viable cells are quantified by measuring the strength of the fluorescence signal using SoftMax Pro (excitation at 560 nm and emission at 590 nm). IC50 values are derived using a 9-point curve fitted with Igor Pro and are presented as mean values from experiments performed in duplicate. Variability between individual values is less than 2-fold.(Only for Reference) |
別名 | RXDX-105, CEP 32496, CEP-32496, CEP32496 |
分子量 | 517.46 |
分子式 | C24H22F3N5O5 |
CAS No. | 1188910-76-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 9 mg/mL (17.39 mM)
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Agerafenib 1188910-76-0 Angiogenesis Apoptosis Cytoskeletal Signaling MAPK Tyrosine Kinase/Adaptors Raf c-RET c-Kit Src PDGFR Bcr-Abl RXDX 105 RXDX-105 Inhibitor Raf kinases inhibit CEP 32496 CEP-32496 CEP32496 RXDX105 inhibitor