Powder: -20°C for 3 years | In solvent: -80°C for 1 year
CHIR-98014 (CT98014) is a selective GSK3 inhibitor; potentiate insulin activation of glucose transport and utilization in vitro and in vivo.
パッケージサイズ | 在庫状況 | 単価(税別) | |||
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無料サンプル 1 mg | |||||
5 mg | 在庫あり | ¥ 16,000 | |||
10 mg | 在庫あり | ¥ 25,000 | |||
25 mg | 在庫あり | ¥ 52,500 | |||
50 mg | 在庫あり | ¥ 85,500 | |||
100 mg | 在庫あり | ¥ 137,000 | |||
500 mg | 在庫あり | ¥ 289,500 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 17,000 |
説明 | CHIR-98014 (CT98014) is a selective GSK3 inhibitor; potentiate insulin activation of glucose transport and utilization in vitro and in vivo. |
ターゲット&IC50 | GSK-3α:0.65 nM, GSK-3β:0.58 nM |
キナーゼ試験 | Kinase assays: Polypropylene 96-well plates are ?lled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing kinase, peptide substrate, and any activators. CHIR-98014 or controls are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a ?nal concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed ?ve times with PBS, ?lled with 200 μL of scintillation ?uid, sealed, left 30 min, and counted in a scintillation counter. All steps are performed at room temperature. |
細胞研究 | CHO-IR cells expressing human insulin receptor are grown to 80% con?uence in Hamm’s F12 medium with 10% fetal bovine serum and without hypoxanthine. Trypsinized cells are seeded in 6-well plates at 1 × 106 cells/well in 2 mL of medium without fetal bovine serum. After 24 hours, medium is replaced with 1 mL of serum-free medium containing GSK-3 inhibitor CHIR 98014 or control (?nal DMSO concentration 0.1%) for 30 min at 37 °C. Cells are lysed by freeze/thaw in 50 mM tris (pH 7.8) containing 1 mM EDTA, 1 mM DTT, 100 mM NaF, 1 mM phenylmethylsulfonyl ?uoride, and 25 μg/mL leupeptin (buffer A) and centrifuged 15 min at 4 °C/14000 g. The activity ratio of GS is calculated as the GS activity in the absence of glucose-6-phosphate divided by the activity in the presence of 5 mM glucose-6-phosphate. (Only for Reference) |
別名 | CHIR 98014, CT98014, CHIR98014 |
分子量 | 486.31 |
分子式 | C20H17Cl2N9O2 |
CAS No. | 252935-94-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 8 mg/mL (16.45 mM), warmed
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
CHIR-98014 252935-94-7 Angiogenesis MAPK PI3K/Akt/mTOR signaling Stem Cells Tyrosine Kinase/Adaptors S6 Kinase Src FGFR GSK-3 Glycogen synthase kinase-3 CT-98014 CHIR 98014 Inhibitor CT98014 CHIR98014 inhibit CT 98014 Glycogen synthase kinase 3 inhibitor