Dexamethasone

カタログ番号 T1076 CAS 50-02-2

別名: MK 125, Prednisolone F, NSC 34521, Hexadecadrol

Dexamethasone (Hexadecadrol) is a glucocorticoid receptor agonist and an IL receptor modulator. Dexamethasone has anti-inflammatory and immunosuppressive activity and induces autophagy. Dexamethasone inhibits LPS-induced inflammatory responses in macrophages.

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Dexamethasone, CAS 50-02-2

パッケージサイズ	在庫状況	単価(税別)
サンプルについてお問い合わせ
500 mg	在庫あり	￥ 9,500
1 mL * 10 mM (in DMSO)	在庫あり	￥ 10,500

バルクのお問い合わせ

バッチを選択

純度: 99.91%

COA DATASHEET SDS HNMR HPLC

純度: 99.77%

COA DATASHEET SDS

純度: 99.77%

COA DATASHEET SDS HNMR HPLC

純度: 98%

COA DATASHEET SDS HNMR

COA DATASHEET SDS HNMR HPLC

バッチの詳細情報はお問い合わせください

生物学的特性に関する説明

化学的特性

保存条件 & 溶解度情報

説明	Dexamethasone (Hexadecadrol) is a glucocorticoid receptor agonist and an IL receptor modulator. Dexamethasone has anti-inflammatory and immunosuppressive activity and induces autophagy. Dexamethasone inhibits LPS-induced inflammatory responses in macrophages.
In vitro	METHODS: Human colorectal cancer cells LoVo and HCT116 were treated with Dexamethasone (10-300 µM) for 72 h. Cell growth inhibition was detected by MTT. RESULTS: Dexamethasone dose-dependently inhibited the growth of LoVo and HCT116 cells, and the inhibition rates of 300 µM Dexamethasone were 52.6% and 58.8%, respectively. [1] METHODS: Acute lymphoblastic leukemia cells RS4;11 were treated with Dexamethasone (100 nM) for 24-36 h. Cell morphology was examined using electron microscopy. RESULTS: In cells treated with Dexamethasone for 24 h, vesicles were surrounded by double membranes, which are characteristic of autophagosomes, and contained membrane structures and/or part of the endoplasmic reticulum or a large amount of cytoplasm. In addition to the appearance of autophagosomes, the nucleus and cell morphology were initially intact, suggesting that autophagosome formation preceded cell death. Dexamethasone induces autophagy. [2] METHODS: Activated mouse and human T cells were treated with Dexamethasone (0.001-10 μM) for 48 h, and PD-1 expression was detected by Flow Cytometry. RESULTS: Dexamethasone enhanced the expression of PD-1 in mouse and human activated T cells. [3]
In vivo	METHODS: To investigate the anti-inflammatory effects, Dexamethasone (1-10 mg/kg) was administered as a single intraperitoneal injection to LPS-induced inflammation in C57Bl/6JBom mice. RESULTS: 10 mg/kg Dexamethasone significantly reduced neutrophils in bronchoalveolar lavage fluid.Dexamethasone treatment significantly down-regulated the levels of TNF-α, IL-1α, IL-1β, IL-6, IL-12p40 and MIP-1α mRNA. Dexamethasone exerts anti-inflammatory and antioxidant functions in acute airway inflammation. [4] METHODS: To detect anti-tumor activity in vivo, Dexamethasone (1 mg/kg) was intraperitoneally injected into SCID mice harboring the human myeloma tumor OPM2 five days per week for three weeks. RESULTS: Dexamethasone treatment significantly inhibited the growth of OPM2 tumors, indicating antitumor activity in vivo. [5]
動物実験	NAC was administered at three different doses (10, 100 and 500 mg/kg body weight). At the highest concentration, the acidic pH of the NAC solution was adjusted by adding NaOH. Dexamethasone was administered as a single injection of 1 or 10 mg/kg. Both drugs were dissolved in saline and 400 μl were injected intraperitoneally, either 1 h before or 1 h after LPS exposure. In one experiment, NAC (100 and 500 mg/kg) was injected successively every 4·5 h, starting 1 h before challenge (five injections in total). A control group of LPS-exposed animals were injected intraperitoneally with solvent alone (saline). Intratracheal administration was performed by instillation of 100 μl NAC (50, 100 or 500 mg/kg) or dexamethasone (10 mg/kg) into the lungs of mice anaesthetized with 15 mg/kg Rapinovet (i.v.) [4].

別名	MK 125, Prednisolone F, NSC 34521, Hexadecadrol
分子量	392.46
分子式	C22H29FO5
CAS No.	50-02-2

保存条件

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度情報

Ethanol: 6 mg/mL (15.28 mM)

DMSO: 73 mg/mL (186 mM)

参考文献

1. He J, et al. Dexamethasone affects cell growth/apoptosis/chemosensitivity of colon cancer via glucocorticoid receptor α/NF-κB. Oncotarget. 2017 Jun 28;8(40):67670-67683. 2. Laane E, et al. Cell death induced by dexamethasone in lymphoid leukemia is mediated through initiation of autophagy. Cell Death Differ. 2009 Jul;16(7):1018-29. 3. Xing K, et al. Dexamethasone enhances programmed cell death 1 (PD-1) expression during T cell activation: an insight into the optimum application of glucocorticoids in anti-cancer therapy. BMC Immunol. 2015 Jun 26;16:39. 4. Rocksén D, et al. Differential anti-inflammatory and anti-oxidative effects of dexamethasone and N-acetylcysteine in endotoxin-induced lung inflammation. Clin Exp Immunol. 2000 Nov;122(2):249-56. 5. Kervoëlen C, et al. Dexamethasone-induced cell death is restricted to specific molecular subgroups of multiple myeloma. Oncotarget. 2015 Sep 29;6(29):26922-34. 6. Huang L, Li Q. Notoginsenoside R1 promotes differentiation of human alveolar osteoblasts in inflammatory microenvironment through inhibiting NF‑κB pathway and activating Wnt/β‑catenin pathway[J]. Molecular Medicine Reports. 2020, 22(6): 4754-4762. 7. Zhang, Depeng, et al. Tabersonine attenuates lipopolysaccharide-induced acute lung injury via suppressing TRAF6 ubiquitination[J]. Biochemical pharmacology. 2018 Aug;154:183-192. 8. Sun C, Li X, Guo E, et al. MCP-1/CCR-2 axis in adipocytes and cancer cell respectively facilitates ovarian cancer peritoneal metastasis[J]. Oncogene. 2019: 1-15. 9. Li M, Yu H. Identification of WP1066, an inhibitor of JAK2 and STAT3, as a Kv1. 3 potassium channel blocker[J]. . British Journal of Pharmacology. 2021

引用文献

1. Liu Z, Tan S, Zhou L, et al.SCGN deficiency is a risk factor for autism spectrum disorder.Signal Transduction and Targeted Therapy.2023, 8(1): 1-15. 2. Chen S, Zhou X, Li W, et al.Development of a novel peptide targeting GPR81 to suppress adipocyte-mediated tumor progression.Biochemical Pharmacology.2023: 115800. 3. Xiong W, Jia L, Liang J, et al.Investigation into the anti-airway inflammatory role of the PI3Kγ inhibitor JN-PK1: An in vitro and in vivo study.International Immunopharmacology.2022, 111: 109102. 4. Wang C, Chen R, Zhu X, et al.METTL14 alleviates the development of osteoporosis in ovariectomized mice by upregulating m6A level of SIRT1 mRNA.Bone.2022: 116652. 5. Huang L, Li Q. Notoginsenoside R1 promotes differentiation of human alveolar osteoblasts in inflammatory microenvironment through inhibiting NF‑κB pathway and activating Wnt/β‑catenin pathway. Molecular Medicine Reports. 2020, 22(6): 4754-4762 6. Li M, Yu H. Identification of WP1066, an inhibitor of JAK2 and STAT3, as a Kv1. 3 potassium channel blocker. British Journal of Pharmacology. 2021 Jul;178(13):2617-2631. doi: 10.1111/bph.15441. Epub 2021 May 20. 7. Zhang D, Li X, Hu Y, et al. Tabersonine attenuates lipopolysaccharide-induced acute lung injury via suppressing TRAF6 ubiquitination. Biochemical Pharmacology. 2018, 154: 183-192 8. Ding C, Fu S, Chen X, et al. Epigallocatechin gallate affects the proliferation of human alveolar osteoblasts and periodontal ligament cells, as well as promoting cell differentiation by regulating PI3K/Akt signaling pathway. Odontology. 2021 Jul;109(3):729-740. doi: 10.1007/s10266-021-00597-1. Epub 2021 Mar 6. 9. Chen Q Z, Li Y, Shao Y, et al. TGF-β1/PTEN/PI3K signaling plays a critical role in the anti-proliferation effect of tetrandrine in human colon cancer cells. International Journal of Oncology. 2017, 50(3): 1011-1021 10. Tian S, Wang R, Chen S, et al. Structural Basis for PPARs Activation by the Dual PPARα/γ Agonist Sanguinarine: A Unique Mode of Ligand Recognition. Molecules. 2021, 26(19): 6012.

11. Sun C, Li X, Guo E, et al. MCP-1/CCR-2 axis in adipocytes and cancer cell respectively facilitates ovarian cancer peritoneal metastasis. Oncogene. 2019: 1-15. 12. Li B, Zhou Q, Wan Q, et al.EZH2 K63-polyubiquitination affecting migration in extranodal natural killer/T-cell lymphoma.Clinical Epigenetics.2023, 15(1): 187. 13. Hou Z, Ren Y, Zhang X, et al.EP300-ZNF384 transactivates IL3RA to promote the progression of B-cell acute lymphoblastic leukemia.Cell Communication and Signaling.2024, 22(1): 211.

もっと見る隠し

投与量変換

You can also refer to dose conversion for different animals. 詳細

In vivo投与量計算 (透明溶液)

ステップ1: 以下の情報を入力してください

投与量

mg/kg

動物の平均体重

動物あたりの投与量

動物数

溶媒の組成を入力してください

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

計算するリセット

計算結果:

作業濃度: mg/ml;

DMSO溶液の作成方法: mg あらかじめ溶解した薬剤 μL DMSO (マスター溶液の濃度 mg/mL)，濃度がそのバッチの薬剤のDMSO溶解度を超える場合は、まずご連絡ください。

生体内薬剤の調合法：取る μL DMSO マスター溶液、次に追加 μL PEG300，確実に混ぜてから加える μL Tween 80，確実に混ぜてから加える μL ddH₂O, 確実に混ぜる

お問合せ内容:

必ず順番通りに溶媒を加えてください。ボルテックスミキサーや超音波、湯煎することで溶解しやすくなります

計算器

モル濃度計算機

希釈計算機

再構成計算

分子量計算機

質量

濃度

体積

分子量

モル度計算機では以下の計算が可能です

既知の体積と濃度の溶液を調製するために必要な化合物の質量
質量が既知の化合物を目的の濃度まで溶解させるのに必要な溶液の量
特定の体積の中に既知の質量の化合物を入れて得られる溶液の濃度

参考例

モル濃度計算機を使用したモル濃度計算の例
化合物の分子量が197.13g/molである場合、10mlの水に10mMのストック溶液を作るのに必要な化合物の質量はどれくらいですか？
［分子量（MW）］の欄に［197.13］と入力してください
[濃度]ボックスに10と入力し、正しい単位（millimolar）を選択します
[容量]ボックスに10と入力し、正しい単位（milliliter）を選択します
計算を押します
答えの19.713mgが質量欄に表示されます

濃度 (開始)

体積 (開始)

濃度 (終了)

体積 (終了)

溶液を作るのに必要な希釈率の計算

溶液の調製に必要な希釈率の算出
希釈計算機は、既知の濃度の原液をどのように希釈するかを計算することができる便利なツールです。V1を計算するためにC1、C2＆V2を入力します。

参考例

Tocrisの希釈計算器を用いた希釈計算の一例
50μMの溶液を20ml作るためには、10mMの原液を何ml必要ですか？
C1V1=C2V2という式を用いて、C1=10mM、C2=50μM、V2=20ml、V1を未知数とします。
濃度（開始）ボックスに10を入力し正しい単位(millimolar)を選択してください
濃度（終了）ボックスに50を入力し正しい単位(millimolar)を選択してください
体積（終了）ボックスに20を入力し正しい単位(millimolar)を選択してください
計算を押します
100 microliter (0.1 ml) という答えが体積（開始）ボックスに表示されます。

分子式

分子量 g/mol

化合物の化学式を入力して、そのモル質量や元素組成を計算します

Tヒント：化学式は大文字と小文字を区別します。: C10H16N2O2 c10h16n2o2

化合物のモル質量（分子量）を計算する手順:
化学物質のモル質量を計算するには、その化学式を入力し、「計算」をクリックしてください。.
分子質量、分子量、モル質量、モル重量の定義:
分子質量（分子量）とは、物質の1分子の質量であり、統一された原子質量単位（u）で表されます。(1uは炭素12の1原子の質量の1/12に等しい）
モル質量（molar weight）とは、ある物質の1モルの質量のことで、単位はg/molです。

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技術サポート

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Dexamethasone 50-02-2 Autophagy Endocrinology/Hormones Immunology/Inflammation Microbiology/Virology IL Receptor Mitophagy Glucocorticoid Receptor SARS-CoV Antibiotic Antibacterial Complement System receptor SARS coronavirus inhibit MK 125 Mitochondrial Autophagy CD18 Prednisolone F CD62L glucocorticoid Inhibitor NSC34521 monocytes NSC 34521 neutrophils Bacterial MK-125 NSC-34521 Hexadecadrol MK125 CD11b inhibitor

本ウェブサイトに掲載されている製品は全て研究用試薬です。研究目的以外の用途にはご使用できません。

Dexamethasone

保存条件

溶解度情報

参考文献

引用文献

関連化合物ライブラリー

関連製品

投与量変換

In vivo投与量計算 (透明溶液)

計算器

モル度計算機では以下の計算が可能です

溶液を作るのに必要な希釈率の計算

バイアルを再構成するのに必要な溶媒の量を計算する.

化合物の化学式を入力して、そのモル質量や元素組成を計算します

技術サポート

Keywords