Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Hydroxyfasudil Hydrochloride (HA 1100 hydrochloride) is a ROCK1/2 inhibitor (IC50s: 0.73/0.72 μM).
パッケージサイズ | 在庫状況 | 単価(税別) | |||
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2 mg | 在庫あり | ¥ 8,000 | |||
5 mg | 在庫あり | ¥ 13,000 | |||
10 mg | 在庫あり | ¥ 19,500 | |||
25 mg | 在庫あり | ¥ 29,500 | |||
50 mg | 在庫あり | ¥ 35,500 | |||
100 mg | 在庫あり | ¥ 66,000 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 14,000 |
説明 | Hydroxyfasudil Hydrochloride (HA 1100 hydrochloride) is a ROCK1/2 inhibitor (IC50s: 0.73/0.72 μM). |
ターゲット&IC50 | ROCK1:0.73 μM, ROCK2:0.72 μM |
In vitro | Hydroxyfasudil has a weak inhibition of PKA (IC50: 37 μM), 50-fold higher than those of the ROCKs. Hydroxyfasudil increases eNOS mRNA levels (EC50: 0.8 ± 0.3 μM). Hydroxyfasudil (0-100 μM) concentration-dependently increases eNOS activity and stimulates NO production in HAEC. Hydroxyfasudil (10 μM) increases the half-life of eNOS mRNA from 13 to 16 hours but does not affect eNOS promoter activity (0.1-100 μM). |
In vivo | Hydroxyfasudil (10 mg/kg, i.p.) markedly increases both the maximal and average voided volumes in SD rats. Hydroxyfasudil also markedly reduces the maximal detrusor pressure. In spontaneously hypertensive rats (SHRs), Hydroxyfasudil (3 mg/kg, i.p.) inhibits hypercontractility induced by norepinephrine. Additionally, Hydroxyfasudil (3/10 mg/kg, i.p.) significantly ameliorates decreased penile cGMP contents in rats. |
動物実験 | Hydroxyfasudil is formulated in saline.Micturition behavior is studied after administration of either Hydroxyfasudil (10 mg/kg, i.p.) or a corresponding volume of saline. Each rat is placed in a metabolic cage containing a urine collection funnel that is placed over an electronic balance. The balance is connected to a personal computer via a multiport controller and used to measure the cumulative weight of the collected urine. Every 150 s during a continuous 24-h period, the computer samples and records the data for the micturition frequency and volumes. The micturition reflex parameters that are collected include: urine volume per micturition, maximal micturition volume, micturition frequency, and total urine output in the Hydroxyfasudil- or vehicle-treated animals. Each monitoring session started at 18.00 hours. Prior to being placed in the metabolic cage at the start of each experimental period, the animals received either a single injection of Hydroxyfasudil (10 mg/kg) dissolved in saline or an injection of saline without the inhibitor. |
別名 | Hydroxyfasudil (HA-1100) HCl, HA 1100 hydrochloride |
分子量 | 343.83 |
分子式 | C14H18ClN3O3S |
CAS No. | 155558-32-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 62 mg/mL(180.3 mM)
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Hydroxyfasudil Hydrochloride 155558-32-0 Cell Cycle/Checkpoint Cytoskeletal Signaling Stem Cells ROCK Hydroxyfasudil (HA-1100) HCl HA-1100 Hydroxyfasudil Rho-associated kinase HA 1100 inhibit HA1100 HA1100 Hydrochloride Rho-kinase Rho-associated protein kinase HA-1100 Hydrochloride ROK HA 1100 hydrochloride Inhibitor HA 1100 Hydrochloride inhibitor