Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PF-4708671 is a cell-permeable inhibitor of p70 ribosomal S6 kinase (S6K1 isoform) .In cell-free assays, PF-4708671(PF4708671) is potent for S6K1(Ki50=20 nM, IC50=160 nM).
パッケージサイズ | 在庫状況 | 単価(税別) | |||
---|---|---|---|---|---|
サンプルについてお問い合わせ | |||||
5 mg | 在庫あり | ¥ 11,000 | |||
10 mg | 在庫あり | ¥ 16,000 | |||
25 mg | 在庫あり | ¥ 26,000 | |||
50 mg | 在庫あり | ¥ 35,500 | |||
100 mg | 在庫あり | ¥ 64,000 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 18,000 |
説明 | PF-4708671 is a cell-permeable inhibitor of p70 ribosomal S6 kinase (S6K1 isoform) .In cell-free assays, PF-4708671(PF4708671) is potent for S6K1(Ki50=20 nM, IC50=160 nM). |
ターゲット&IC50 | p70 S6K1:160 nM |
In vivo | PF-4708671 inhibits the phosphorylation of S6 protein mediated by S6K1 in response to IGF-1 (Insulin-like Growth Factor 1) and can also induce the phosphorylation of the T-loop and hydrophobic motif of S6K1 through mTORC1 (mTOR complex 1). |
キナーゼ試験 | Affinity determination: Purified activated FAK kinase domain (amino acids 410–689) is reacted with 50 μM ATP, and 10 μg/well of a random peptide polymer of Glu and Tyr (molar ratio of 4:1), poly(Glu/Tyr) in kinase buffer (50 mM HEPES, pH 7.5, 125 mM NaCl, 48 mM MgCl2) for 15 min. Phosphorylation of poly(Glu/Tyr) is challenged with serially diluted compounds at 1/2-Log concentrations starting at a top concentration of 1 μM. Each concentration is run in triplicate. Phosphorylation of poly(Glu/Tyr) is detected with a general anti-phospho-tyrosine (PY20) antibody, followed by horseradish peroxidase-conjugated goat anti-mouse IgG antibody. The standard horseradish peroxidase substrate 3, 3', 5, 5'-tetramethylbenzidine is added, and Optical Density readings at 450 nm are obtained following the addition of stop solution (2 M H2SO4). The IC50 values are determined using the Hill slope model. |
別名 | PF4708671 |
分子量 | 390.41 |
分子式 | C19H21F3N6 |
CAS No. | 1255517-76-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 19.5 mg/mL (50 mM)
Ethanol: 19.5 mg/mL (50 mM)
You can also refer to dose conversion for different animals. 詳細
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PF-4708671 1255517-76-0 Autophagy MAPK PI3K/Akt/mTOR signaling S6 Kinase inhibit S6K Inhibitor PF4708671 Ribosomal S6 Kinase (RSK) PF 4708671 inhibitor