Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PP-121 is a multi-targeted inhibitor of PDGFR (IC50: 2 nM), Hck (IC50: 8 nM), mTOR (IC50: 10 nM), VEGFR2(IC50: 12 nM), Src (IC50: 14 nM) and Abl (IC50: 18 nM) , also inhibits DNA-PK (IC50: 60 nM).
パッケージサイズ | 在庫状況 | 単価(税別) |
---|---|---|
5 mg | お問い合わせ | ¥ 12,000 |
10 mg | お問い合わせ | ¥ 18,000 |
25 mg | お問い合わせ | ¥ 36,000 |
50 mg | お問い合わせ | ¥ 57,500 |
100 mg | お問い合わせ | ¥ 83,500 |
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 12,000 |
説明 | PP-121 is a multi-targeted inhibitor of PDGFR (IC50: 2 nM), Hck (IC50: 8 nM), mTOR (IC50: 10 nM), VEGFR2(IC50: 12 nM), Src (IC50: 14 nM) and Abl (IC50: 18 nM) , also inhibits DNA-PK (IC50: 60 nM). |
ターゲット&IC50 | mTOR:10 nM, Hck:8 nM, PDGFR:2 nM, Abl:18 nM, VEGFR2/KDR:12 nM, Src:14 nM |
キナーゼ試験 | Kinase assays: Purified kinase domains are incubated with PP-121 at 2- or 4-fold dilutions over a concentration range of 1 nM-50 μM or with vehicle (0.1% DMSO) in the presence of 10 μM ATP, 2.5 μCi of γ-32P-ATP and substrate. Reactions are terminated by spotting onto nitrocellulose or phosphocellulose membranes, depending on the substrate; this membrane is then washed 5–6 times to remove unbound radioactivity and dried. Transferred radioactivity is quantitated by phosphorimaging and IC50 values are calculated by fitting the data to a sigmoidal doseresponse using Prism software. |
細胞研究 | For western blot analysis, cells are grown in 12-well plates and treated with PP-121 at the indicated concentrations or vehicle (0.1% DMSO). Treated cells are lysed, lysates are resolved by SDS-PAGE, transferred to nitrocellulose and blotted. For cell proliferation assays,cells are grown in 96-well plates are treated with PP-121 at 4-fold dilutions (10 μM - 0.040 μM) or vehicle (0.1% DMSO). After 72 hours cells are exposed to Resazurin sodium salt (22 μM) and fluorescence is quantified. IC50 values are calculated using Prism software. For proliferation assays involving single cell counting, non-adherent cells are plated at low density (3–5% confluence) and treated with PP-121 (2.5 μM) or vehicle (0.1% DMSO). Cells are diluted into trypan blue daily and viable cells counted using a hemocytometer. For apoptosis and cell cycle analysis, cells are treated with the indicated concentration of PP-121 or vehicle (0.1% DMSO) for 24–72 hours. Cells are either stained live with AnnexinV-FITC or fixed with ethanol and stained with propidium iodide. Cell populations are separated using a FacsCalibur flow cytometer; data is collected using CellQuest Pro software and analyzed with either ModFit or FlowJo Software.(Only for Reference) |
分子量 | 319.36 |
分子式 | C17H17N7 |
CAS No. | 1092788-83-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 2 mg/mL (6.26 mM)
DMSO: 60 mg/mL (187.9 mM)
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
PP121 1092788-83-4 Angiogenesis Apoptosis Cytoskeletal Signaling PI3K/Akt/mTOR signaling Tyrosine Kinase/Adaptors VEGFR Hck Bcr-Abl PDGFR Src mTOR Platelet-derived growth factor receptor PP 121 PP-121 inhibit Inhibitor Mammalian target of Rapamycin Vascular endothelial growth factor receptor inhibitor