SJ000291942 is an activator of the canonical bone morphogenetic proteins (BMP) signaling pathway.
| 説明 | SJ000291942 is an activator of the canonical bone morphogenetic proteins (BMP) signaling pathway. |
| In vitro | Embryos treated with SJ000291942 display the most severe ventralization and SJ000291942 is also the most potent. SJ000291942 also causes more mortality with lower doses than controls and the other two compounds. This demonstrates our compounds cause ventralization of embryos consistent with increased BMP signaling activity. SJ000291942 causes an increase in bmp2b and szl expression. Zebrafish assays suggest that SJ000291942 activates the canonical BMP signaling pathway. To extend these observations, immunoblotting of protein lysates from C33A-2D2 cells stimulated with SJ000291942 at different times is performed. SJ000291942 activates phosphorylation of SMAD1/5/8 in serum-free medium. Like in zebrafish embryos, SJ000291942 is most active. SJ000291942 induces p-SMAD1/5/8 maximally at 1hr of treatment. Immunoblotting analysis of lysates from C33A-2D2 treated with SJ000291942 reveals clear induction of the phosphorylated Extracellular Signal-regulated protein Kinase, ERK1/2 (P-ERK1/2) by SJ000291942. The highest dose (100 and 300ng) BMP4 treatments generate a gene expression signature most similar to osteoblast expression. Low dose (10ng) BMP4 treatment aligns closely with 25μM compound 3 treatment and with 25μM SJ000291942. |
| 分子量 | 318.3 |
| 分子式 | C16H15FN2O4 |
| CAS No. | 425613-09-8 |
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
DMSO: 150 mg/mL
( < 1 mg/ml refers to the product slightly soluble or insoluble )
参考文献
投与量変換You can also refer to dose conversion for different animals. 詳細
In vivo投与量計算 (透明溶液)計算器bottom
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SJ000291942 425613-09-8 Stem Cells TGF-beta/Smad Transforming growth factor beta receptors SJ 000291942 SJ-000291942 TGF-β Receptor inhibit Inhibitor inhibitor
