Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Vistusertib (AZD2014) is an orally bioavailable inhibitor of the mammalian target of rapamycin (mTOR) with potential antineoplastic activity.
パッケージサイズ | 在庫状況 | 単価(税別) | |||
---|---|---|---|---|---|
無料サンプル 1 mg | |||||
2 mg | 在庫あり | ¥ 10,000 | |||
5 mg | 在庫あり | ¥ 16,000 | |||
10 mg | 在庫あり | ¥ 27,500 | |||
25 mg | 在庫あり | ¥ 42,500 | |||
50 mg | 在庫あり | ¥ 57,500 | |||
100 mg | 在庫あり | ¥ 91,500 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 17,500 |
説明 | Vistusertib (AZD2014) is an orally bioavailable inhibitor of the mammalian target of rapamycin (mTOR) with potential antineoplastic activity. |
ターゲット&IC50 | pS6 (S235/236):200 nM, mTOR:2.8 nM, P-Akt (S473):80 nM |
キナーゼ試験 | Recombinant truncated FLAG-tagged mTOR expressed in HEK 293 cells is used in biochemical assays, together with a biotinylated p70S6K peptide substrate. Streptavidin donor and protein A acceptor beads are used to assemble the capture complex for generation of the assay signal. The activity of the lipid kinases, PI3K alpha, beta, delta, and gamma are measured using recombinant proteins and the lipid PIP2 as substrate. Assays for ATM and DNA-PK activity are performed. The mTOR cellular activity is measured in MDAMB468 cells, using an Acumen laser scanning cytometer to analyze the levels of phosphorylation of S6 (Ser235/236) and AKT (Ser473)[1]. |
細胞研究 | AZD2014 is prepared in DMSO (10 mM) and stored under nitrogen, and then diluted with appropriate media before use[1]. Cells are plated in 96-well plates for the indicated time. For CellTiterGlo assays: CellTiterGlo is mixed with the cells. Cells are normalized to day 0 control and net growth is determined using the following formula: ((x?y)/(z?y))=net growth, where x=reading of treated sample at end of study, y=average reading on day 0, and z=reading of DMSO-treated sample at end of study. The concentration of DMSO does not exceed 0.03% for any experiment. For MTS assays: adherent cell lines are grown in 96-well plates. MTS reagent is added on day 0 and on day 3 post-AZD2014 addition. Suspension lines are assayed using the Alamar Blue reagent, 72 hours after AZD2014 addition[1]. |
別名 | AZD2014 |
分子量 | 462.54 |
分子式 | C25H30N6O3 |
CAS No. | 1009298-59-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 36 mg/mL (77.8 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Vistusertib 1009298-59-2 Apoptosis Autophagy Cytoskeletal Signaling MAPK PI3K/Akt/mTOR signaling Akt PI3K mTOR S6 Kinase AZD2014 inhibit AZD-2014 Mammalian target of Rapamycin AZD 2014 Inhibitor inhibitor