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Phosphatase Inhibitor Cocktail II (100× DMSO)

Catalog No. C0003 Animal-derived-free(for general use)

It is easy for the proteins to be degraded or dephosphorylated during in vitro extraction procedure, which could cause an inaccurate result of protein expression detection. Therefore, adding protease inhibitors or phosphatase inhibitors into the extracts would be an effective method to prevent degradation and dephosphorylation of protein.

The phosphatase inhibitor mixture can effectively inhibit the dephosphorylation of common phosphatases on proteins and maintain the original phosphorylation state of proteins.

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Phosphatase Inhibitor Cocktail II (100× DMSO)
パッケージサイズ 在庫状況 単価(税別)
1 mL 在庫あり ¥ 8,000
1 mL * 10 在庫あり ¥ 56,500
1 mL * 50 在庫あり ¥ 229,000
1 mL * 100 在庫あり ¥ 342,500
ご確認事項

1. 1研究室・グループあたり最大5製品までお申し込みいただけます。 同一製品は1回のみとなります。

2. 1回につき最大2製品までのお申し込みが可能です。

3. 2回目以降をご希望の際は、前回ご提供のサンプルの実験結果をオンラインでご提供いただく必要がございます。

4. 2023 年 1 月 20 日より前にサンプルをお申し込みいただいたお客様は、2023 年の無料申請枠にはカウントされませんが、以前の実験結果をご提供いただく必要があります。

ご確認事項

1. 1研究室・グループあたり最大5製品までお申し込みいただけます。 同一製品は1回のみとなります。

2. 1回につき最大2製品までのお申し込みが可能です。

3. 2回目以降をご希望の際は、前回ご提供のサンプルの実験結果をオンラインでご提供いただく必要がございます。

4. 2023 年 1 月 20 日より前にサンプルをお申し込みいただいたお客様は、2023 年の無料申請枠にはカウントされませんが、以前の実験結果をご提供いただく必要があります。

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Composition

Ingredient CAS MW Concentration (100x) Target Type
(-)-p-Bromotetramisole oxalate 62284-79-1 373.22 2.5 mM Alkaline phosphatases Irreversible
Cantharidin 56-25-7 196.2 500 μM Ser/Thr phosphatases Reversible
Microcystin LR 101043-37-2 995.17 500 nM Acid and PP1 and PP2A Reversible

Features

  • Accurate Ingredients —— Clearance of components and concentrations to avoid experimental uncertainty
  • Comprehensive Protection—— Effectively inhibit various proteases to protect proteins from being degraded
  • Cost-Effective —— Lower price with higher quality

Handling Instruction

  • Compatible with Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, kinase assay and etc.
  • Add concentrated cocktail at 1:100 (v/v) dilution to samples solution (such as cell lysates or tissue extracts) before assaying.
  • Briefly vortex cocktails to help facilitate the dissolution.

Notice

  • Following initial thaw, aliquot and freeze (-20°C) to avoid thawing and refreezing repeatedly.
  • If Cocktail I and Cocktail II are used together, do not mix tube I with tube II beforehand, as there may be precipitation. To avoid this, please add them step by step during experiment.

引用文献

1. Li Y, Xu J, Guo X, et al.The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model.Genome Biology.2023, 24(1): 20. 2. Li B, Yang T, Liu J, et al.Genetically engineered PD-1 displaying nanovesicles for synergistic checkpoint blockades and chemo-metabolic therapy against non-small cell lung cancer.Acta Biomaterialia.2023, 161: 184-200. 3. Li J, Dang P, Li Z, et al.Peroxisomal ERK mediates Akh/glucagon action and glycemic control.Cell Reports.2023, 42(10). 4. Wu K, Zhang Y, Liu Y, et al.Phosphorylation of UHRF2 affects malignant phenotypes of HCC and HBV replication by blocking DHX9 ubiquitylation.Cell Death Discovery.2023, 9(1): 27. 5. Li W, Liang J, Li S, et al.The CXCL12-CXCR4-NLRP3 axis promotes Schwann cell pyroptosis and sciatic nerve demyelination in rats.Clinical and Experimental Immunology.2023: uxad081. 6. Tang H, Guo J, Sang B, et al. PDGFRβ Modulates Aerobic Glycolysis in Osteosarcoma HOS Cells via the PI3K/AKT/mTOR/c-Myc Pathway. Biochemistry and Cell Biology. 2021 (ja). 7. Zhang Y, Zhang J, Chen Y, et al. The ORF8 Protein of SARS-CoV-2 Mediates Immune Evasion through Potently Downregulating MHC-I. Proceedings of the National Academy of Sciences. 2021, 118(23). 8. Wang W, Luo X, Zhang Q, et al. Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research. 2020, 26: e920583-1. 9. Tong Y, Liu L, Wang R, et al. Berberine Attenuates Chronic Atrophic Gastritis Induced by MNNG and Its Potential Mechanism. Frontiers in Pharmacology. 2021, 12: 382 10. Wang L, Deng K, Gong L, et al. Chlorquinaldol targets the β-catenin and T-cell factor 4 complex and exerts anti-colorectal cancer activity. Pharmacological Research. 2020: 104955.
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